GBS

As a licensed Genotyping-by-sequencing (GBS) service provider, the Genomics Center provides cost-effective, high-throughput genotyping to academic and industry scientists world-wide. GBS has gained popularity as a robust, genome-wide SNP discovery method with particular utility for species for which there are no commercially-available targeted reagents.

There are a number of technical variants to reduced representation sequencing, including Genotyping-by-sequencing (GBS), Sequence-Based Genotyping (SBG), Restriction Site-Associated DNA Sequencing (RAD-Seq), and Double Digest RAD-Seq (ddRAD-Seq). However, in all of these methods, the general principle is the same: genomic DNA is digested with restriction enzyme(s), ligated with complementary adaptors, amplified, sequenced, and analyzed for SNP genotypes.

Because the same reduced representation of the genome is sequenced from sample to sample, only a small amount of sequencing is needed to provide sufficient coverage for reliable SNP calls.

Key Features of Our Method

• For species new to GBS: we offer pilot projects to validate a restriction enzyme on a sub-set of samples to assess the number of informative markers before committing to a full GBS project.
• Complete sample-to-genotyping workflow: DNA extraction, GBS library creation, sequencing, and variant calling.
• Multiplex capabilities up to 3,200-plex: this is ideal for the production-scale NovaSeq X Plus to give unprecedented sequencing turnaround and low-costs.
• High-quality genotyping: > 95% genotyping rate per locus achievable.

Questions

Whether your species is new to GBS or has an established protocol, we can help determine which sequencing instrument will best meet your budget and project goals. Clients are encouraged to contact our experienced GBS team for a consult on experimental set-up and sequencing options at gbs@umn.edu. Please tell us about your project by filling out our GBS project inquiry form to start your project today.