Quantification of nucleic acids can be achieved via UV spectroscopy or via the use of fluorometry (in combination with DNA (PicoGreen) or RNA (RiboGreen)-binding dyes).
The NanoDrop instrument for UV quantification consumes only 1-2 ul of material, and provides a measure of DNA purity (260/280 and 260/230 ratios), but is not able to accurately quantify impure nucleic acids or DNA or RNA at concentrations below ≈ 10 ng/ul.
PicoGreen and RiboGreen quantification are therefore the gold standards for sensitive and specific quantification of DNA and RNA, respectively.
Questions
Please contact our QC team at umgc-qc@umn.edu for more information and questions regarding quantification.
UMN Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| NanoDrop Quantification. | $2.12 | $3.31 | $6.55 |
| Turnaround time. | 4 to 5 days | 2 to 3 days | 1 to 1 days |
| PicoGreen DNA Quantification. | $2.16 | $8.23 | $10.97 |
| Turnaround time. | 5 to 10 days | 2 to 4 days | 1 to 2 days |
| RiboGreen RNA Quantification. | $2.19 | $8.27 | $11.01 |
| Turnaround time. | 5 to 8 days | 2 to 4 days | 1 to 2 days |
Notes:
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact umgc-qc@umn.edu to see if this is a current option. More information about Pricing Tiers.
External Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| NanoDrop Quantification. | $2.71 | $4.03 | $7.63 |
| Turnaround time. | 4 to 5 days | 2 to 3 days | 1 to 1 days |
| PicoGreen DNA Quantification. | $2.76 | $9.51 | $12.55 |
| Turnaround time. | 5 to 10 days | 2 to 4 days | 1 to 2 days |
| RiboGreen RNA Quantification. | $2.81 | $9.56 | $12.61 |
| Turnaround time. | 5 to 8 days | 2 to 4 days | 1 to 2 days |
Notes:
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact umgc-qc@umn.edu to see if this is a current option. More information about Pricing Tiers.
Submission
NanoDrop Quantifiation, PicoGreen RNA Quantification, and RiboGreen RNA Quantification
Print and submit the completed submission form with your samples.
Please submit 4 ul of sample per assay in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.). Example: if requesting both NanoDrop and PicoGreen, please submit 8 ul of sample.
For NanoDrop submissions, please also provide 2 ul/sample blanking solution if other than water.
Forms
Samples can be dropped off at one of our laboratory locations.
For RNA samples, please make sure samples are submitted directly to a UMGC staff member so that the samples are stored appropriately. DNA samples can be placed in the indicated freezer.
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus).
- 20 Snyder Hall (St. Paul campus) – self-serve drop-off room.
Deliverables
An Excel report is emailed to client with sample IDs and concentrations.
FAQs
What is the turnaround time?
The UMGC guarantees a turnaround time of 3 days. Often results are sent out within 24 hours of sample submission.