NGS Library Manipulation
It may be desirable for your lab to make libraries outside of the UMGC, either to reduce costs or to implement protocols that are not offered as standard services by the UMGC. In order to help researchers with their own library creation efforts, the UMGC offers a variety of DNA manipulation services “à la carte”. These include DNA shearing, LabChip XT size-selection, and library pooling and quality control.
Questions?
Contact Elyse Froehling: next-gen@umn.edu.
UMN Rates
| DNA Shearing | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Covaris Long (>20 min) Shear. w/ Sizing. | $64.88 | $112.08 | $159.91 |
| Turnaround time. | 5 to 8 days | 3 to 6 days | 2 to 3 days |
| Covaris. w/ Sizing. | $36.71 | $55.07 | $85.15 |
| Turnaround time. | 5 to 8 days | 3 to 6 days | 2 to 3 days |
| DNA Clean-up | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Ampure bead. No QC. | $10.75 | $17.20 | $20.43 |
| Turnaround time. | 3 to 6 days | 2 to 4 days | 1 to 2 days |
Notes
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact next-gen@umn.edu to see if this is a current option. More information about Pricing Tiers.
External Rates
| DNA Shearing | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Covaris Long (>20 min) Shear. w/ Sizing. | $82.77 | $135.26 | $188.43 |
| Turnaround time. | 5 to 8 days | 3 to 6 days | 2 to 3 days |
| Covaris. w/ Sizing. | $46.82 | $67.23 | $100.64 |
| Turnaround time. | 5 to 8 days | 3 to 6 days | 2 to 3 days |
| DNA Clean-up | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Ampure bead. No QC. | $13.73 | $20.88 | $24.48 |
| Turnaround time. | 3 to 6 days | 2 to 4 days | 1 to 2 days |
Notes
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact next-gen@umn.edu to see if this is a current option. More information about Pricing Tiers.
Submission
How to Order
Covaris DNA Shearing
Please print and submit a completed form with your samples:
Covaris Shearing Internal Submission Form
Covaris Shearing External Submission Form
Caliper LabChip XT Size Selection or Library Pooling
Please contact next-gen@umn.edu for project specifications.
Shipping Instructions
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.
Samples can be dropped off at one of our campus locations
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Samples can be shipped to the following address:
Please send the tracking information to next-gen@umn.edu.
UMN Genomics Center
ATTN: Nasia Mead
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736