NGS Library Quality Control

Overview

The UMGC welcomes client-submitted NGS libraries but requires UMGC quality control assessment prior to sequencing. Client NGS library QC includes three bundled assays:

PicoGreen quantification
Quantitative capillary electrophoretic sizing (Agilent)
Functional quantification (KAPA Biosystems Q-PCR)

Questions

Please contact umgc-qc@umn.edu for any questions about library QC.

Pricing

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UMN Rates

Service	Savings Tier	Speed Tier	Priority Tier
KAPA Library QC.	$77.76	$119.46	$168.01
Turnaround time.	7 to 10 days	4 to 6 days	3 to 4 days

_Notes
_{1. Library QC is required for libraries prepared outside the UMGC. This rate includes KAPA QC, PicoGreen, and Agilent sizing.}
_Tiers:_{Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact}_{umgc-qc@umn.edu}_{to see if this is a current option.}_{More information about Pricing Tiers}_.

External Rates

Service	Savings Tier	Speed Tier	Priority Tier
KAPA Library QC.	$99.20	$145.57	$199.54
Turnaround time.	7 to 10 days	4 to 6 days	3 to 4 days

Guidelines

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Submission

How to Order

For all direct sequencing submissions of NGS libraries created outside the UMGC, please contact next-gen@umn.edu.

University researchers submitting NGS libraries for QC only please print and submit the completed submission form with your samples.

Submit 10 ul of sample with a concentration greater than 2 nM per sample in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.).

Forms

QC – UMN Submission Form

QC – External Submission Form

Deliverables

A QC report is emailed to client including library concentration (both ng/ul and nM) and size (Agilent).

FAQ

What is the turnaround time?

The UMGC guarantees a turnaround time of 7 days.